Figure 1.

Altered Autophagy and Apoptosis in Aged MuSCs

MuSCs were isolated throughout a time course of aging and plated for 48 hr for autophagy and apoptosis markers.

(A) Upper: representative images of LC3B punta after 10 μM chloroquine treatment in the final 12 hr of culture. Arrowheads indicate LC3B punta. Lower: quantification of relative punta number within age groups. DAPI, blue; LC3B, green; actin, red (N = 3 independent experiments) (A). Scale bar, 5 μm. Representative western blot of LC3B isoforms I and II after 10 μM chloroquine treatment in the final 12 hr of culture (B).

(C) Total and cleaved PARP protein expression in MuSCs after 48 hr in culture.

(D) Upper: representative TUNEL staining during respective ages. DAPI, blue; TUNEL, green. Scale bar, 50 μm. Lower: quantification of percentage TUNEL-positive MuSCs (N = 4 independent experiments). (E). Upper: representative EdU staining during respective ages. DAPI, blue; EdU, pink. Scale bar, 100 μm. Lower: quantification of percentage EdU-positive MuSCs (N = 3 independent experiments).

^∗ Signifies different from young; † signifies different than old (p < 0.05). Data are presented as means ± SE.