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Alternative splicing of hnRNP genes regulated by PUF60/RBM39. (A) HNRNPK. (B) HNRNPD. (C–F) RT-PCR validation. Down- and up-regulated exonic segments are marked by red and green rectangles at the top, respectively. The siRNA concentrations were 50 and 90 nM. SC, scrambled siRNA controls. Exons (e) targeted by amplification primers (Supplementary Table S1) are at the bottom. Columns show the relative abundance of the indicated transcripts (shown in panels A and B).
