Figure 9.

Alternative splicing of PUF60 affects 3′ss choice. (A) Exon structure of tested PUF60 mRNA isoforms. (B) UBE2E splicing pattern in HEK293 cells individually expressing PUF60 isoforms (upper panel). Their expression was assayed by the anti-myc antibodies (lower panel). NC, no plasmid control, H169Y, a negative control for cr3′ss. Error bars are SDs of two transfections. P-value was derived by an unpaired t-test. (C) Exon usage of UBE2F and OGDH reporters (Figure 7A) cotransfected with plasmids expressing PUF60 isoforms. Error bars, SDs of two transfections.