Table 1.
Proteins and peptides selection for inclusion in quantitative assay. Proteins were chosen based on results from previous studies and are listed based on retention time. SpectrumMill MRM and Skyline were used to choose initial peptides and transitions. Precursor ions (Prec Ion), fragmentation voltage (CEV), Product Ions, and Ion fragments are shown for each peptide. Transitions for endogenous peptides are highlighted in grey, labeled peptides are shown in white.
| Peptide, protein name, accession # | RT | Prec Ion | CE (V) | Product Ion | Ion fragment |
|---|---|---|---|---|---|
| LAQAYYESTR Filaggrin I P20930 | 5.14 | 601.3 | 16.8 | 889.4 | y7 |
| 655.3 | y5 | ||||
| LAQAYYESTR* Filaggrin I P20930 (AQUA) | 606.3 | 16.8 | 899.4 | y7 | |
| 665.3 | y5 | ||||
| IILQPCGSK (alk) Corneodesmosin Q15517 | 6.44 | 508.3 | 13.5 | 789.4 | y7 |
| 548.2 | y5 | ||||
| IILQPCGSK* (alk) Corneodesmosin Q15517 (AQUA) | 512.3 | 13.5 | 797.4 | y7 | |
| 556.3 | y5 | ||||
| TTQFSCTLGEK (alk) Fatty Acid Binding Protein Q01469 | 6.52 | 636.3 | 18.1 | 941.4 | y8 |
| 794.4 | y7 | ||||
| TTQFSCTLGEK* (alk) Fatty Acid Binding Protein Q01469 (AQUA) | 640.3 | 18.1 | 949.5 | y8 | |
| 802.4 | y7 | ||||
| TNSILFYGR Serpin B3 P29508 | 9.65 | 535.8 | 14.5 | 855.5 | y7 |
| 655.4 | y5 | ||||
| TNSILFYGR* Serpin B3 P29508 (AQUA) | 540.8 | 14.5 | 865.5 | y7 | |
| 665.4 | y5 | ||||
| ITWLYDNTTGK Transglutaminase 3 Q08188 | 10.09 | 656.3 | 18.8 | 911.4 | y8 |
| 798.4 | y7 | ||||
| ITWLYDNTTGK* Transglutaminase 3 Q08188 (AQUA) | 660.3 | 18.8 | 919.5 | y8 | |
| 806.4 | y7 | ||||
| ELGVGIALR Fatty Acid Binding Protein Q01469 | 10.49 | 464.3 | 11.9 | 685.4 | y7 |
| 529.3 | y6 | ||||
| ELGVGIALR* Fatty Acid Binding Protein Q01469 (AQUA) | 469.3 | 11.9 | 695.4 | y7 | |
| 539.4 | y6 | ||||
| FYQTSVESVDFANAPEESR Serpin B3 P29508 | 10.7 | 726.0 | 21.3 | 802.4 | y7 |
| 617.3 | y5 | ||||
| FYQTSVESVDFANAPEESR* Serpin B3 P29508 (AQUA) | 729.3 | 21.3 | 812.4 | y7 | |
| 627.3 | y5 | ||||
| GSPGVPSFAAGPPISEGK Corneodesmosin Q15517 | 10.88 | 827.9 | 25 | 755.9 | y16++ |
| 629.3 | y13++ | ||||
| GSPGVPSFAAGPPISEGK* Corneodesmosin Q15517 (AQUA) | 831.9 | 25 | 759.9 | y16++ | |
| 633.3 | y13++ | ||||
| GNPTVEVDLFTSK Alpha enolase P06733 | 11.38 | 703.9 | 20.5 | 938.5 | y8 |
| 618.3 | y11++ | ||||
| GNPTVEVDLFTSK* Alpha enolase P06733 (AQUA) | 707.9 | 20.5 | 946.5 | y8 | |
| 622.3 | y11++ | ||||
| SQGVFQCGPASVIGVR (alk) Transglutaminase 3 Q08188 | 11.78 | 831.4 | 25.1 | 1015.5 | y10 |
| 855.5 | y9 | ||||
| SQGVFQCGPASVIGVR* (alk) Transglutaminase 3 Q08188 (AQUA) | 836.4 | 25.1 | 1025.5 | y10 | |
| 865.5 | y9 | ||||
| AAVPSGASTGIYEALELR Alpha enolase P06733 | 13.56 | 903.0 | 27.7 | 1164.6 | y10 |
| 1063.6 | y9 | ||||
| AAVPSGASTGIYEALELR* Alpha enolase P06733 (AQUA) | 908.0 | 27.7 | 1174.6 | y10 | |
| 1073.6 | y9 | ||||
| SVVTVIDVFYK Filaggrin 2 Q5D862 | 14.9 | 635.4 | 16.8 | 984.5 | y8 |
| 784.4 | y6 | ||||
| SVVTVIDVFYK* Filaggrin 2 Q5D862 AQUA (AQUA) | 639.4 | 16.8 | 992.6 | y8 | |
| 792.4 | y6 | ||||
| NPDDPDMVDVFMDHLDIDHNK Filaggrin I P20930 | 15.43 | 621.3 | 17.6 | 799.9 | y13++ |
| 741.4 | y6 | ||||
| NPDDPDMVDVFMDHLDIDHNK*Filaggrin I P20930 (AQUA) | 623.3 | 17.6 | 803.9 | y13++ | |
| 749.4 | y6 | ||||
| NPDDPDTVDVIMHMLDR Filaggrin 2 Q5D862 | 16.19 | 661.6 | 27.4 | 771.4 | y13++ |
| 623.6 | y16+++ | ||||
| NPDDPDTVDVIMHMLDR* Filaggrin 2 Q5D862 (AQUA) | 665.0 | 27.4 | 776.4 | y13++ | |
| 627.0 | y16+++ |