Table 2. Recovery and RSD values for each of the 14 labeled peptides.

Aliquots of 100 ug pooled sample were spiked with 0.5-25 pmol of labeled peptides. Samples were analyzed in triplicate using an LC/QQQ mass spectrometer system. Data was analyzed as described in methods.

Protein	Filaggrin 1				Corneodesmosin				FABP
Peptide	LAQ..TR		NPD..NK		IIL..SK		GSP.. GK		TTQ..EK		ELG..LR
pmol/100ug protein	Rec.	RSD	Rec.	RSD	Rec.	RSD	Rec.	RSD	Rec.	RSD	Rec.	RSD
0.5	101.3	21.8	115.8	4.2	110.2	1.9	114.3	8.7	119.6	6.4	111.8	2.5
1.25	108.5	3.0	91.1	5.0	93.5	4.8	98.2	4.3	94.2	4.9	93.8	2.2
2.5	94.3	10.0	97.4	3.8	96.4	1.1	92.6	6.8	92.6	2.9	94.2	2.3
5	91.1	11.0	92.6	2.7	98.0	1.8	94.4	3.3	91.3	2.4	96.8	3.5
12.5	105.8	5.3	102.5	3.0	102.1	2.2	97.9	3.4	99.9	3.0	104.3	1.9
25	99.0	6.1	100.6	4.3	99.8	1.6	102.7	2.9	102.4	1.6	99.2	2.8

Protein	Serpin B3				Transglutaminase 3				Alpha Enolase				Filaggrin 2
Peptide	TNS..GR		FYQ..SR		ITW..GK		SQG..VR		GNP..SK		AAV..LR		SVV..YK		NPD..DR
pmol/100ug protein	Rec.	RSD	Rec.	RSD	Rec.	RSD	Rec.	RSD	Rec.	RSD	Rec.	RSD	Rec.	RSD	Rec.	RSD
0.5	120.6	6.0	119.9	8.7	115.7	2.7	115.0	25.3	118.2	11.6	100.1	8.6	107.2	3.3	ND	NC
1.25	89.3	3.9	93.0	14.5	95.3	1.4	91.9	5.4	89.9	5.8	102.1	12.3	95.8	2.1	ND	NC
2.5	91.2	3.2	92.1	3.6	93.7	9.6	96.7	6.8	92.6	5.3	102.5	7.5	96.2	0.8	126.3	63.1
5	94.3	4.1	91.3	2.3	92.0	3.3	96.7	4.0	97.1	3.8	94.1	4.4	98.6	0.5	101.2	21.4
12.5	105.0	1.5	102.5	0.8	102.2	1.6	97.5	2.7	101.5	3.1	100.8	5.0	102.9	2.0	120.7	12.6
25	99.7	1.8	101.2	3.7	101.1	3.4	102.4	4.1	100.7	3.7	100.4	3.0	99.3	1.3	89.1	11.5