Figure 4.
Effects of benzalkonium chloride on 43D growth and MuLV replication. (A) 43D cells were treated with different doses of benzalkonium chloride (BKC) and the cholinium salt of betulinic acid-glycine, BA2 for 48 hrs. The effect of the compounds on cell growth was measured by the AlmarBlue cell proliferation assay. The figure illustrates the relative cell proliferation to the control. The averages of two experiments are shown. (B) 43D cells were treated with 30 μM of the compounds for 48 hrs, and Gag proteins in cells and media were detected by western blots using the rabbit serum for anti-p30CA.