Figure 4.

Protein phosphatase 2A dephosphorylation of TFE3 and TFEB. A, immunoblot analysis of protein lysates from ARPE19 cells treated with the indicated concentrations of FTY720 for 1 h. B, ARPE19 cells treated with vehicle or 100 μm C2-ceramide for 2 h were fixed, permeabilized, and immunostained with antibodies against TFE3. Scale bar, 10 μm. C, immunoblot analysis of protein lysates from ARPE19 cells treated with the indicated concentrations of C2-ceramide or 250 nm Torin-1 for 2 h. Immunoblots are representative of three independent experiments.