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. 2018 Jun 26;293(32):12525–12534. doi: 10.1074/jbc.RA118.003471

Figure 5.

Figure 5.

Protein phosphatase 2A dephosphorylates TFEB and TFE3 in vitro. A, in vitro dephosphorylation of TFEB with ARPE19 cell lysates in the presence of 400 nm okadaic acid. B, in vitro dephosphorylation of TFEB with ARPE19 cell lysates treated with 400 nm okadaic acid for 1 h. C and D, in vitro dephosphorylation of TFEB with ARPE19 cell lysates depleted of PP2A subunits. E, immunoblot analysis of protein lysates from ARPE19 cells depleted of either PPP2A catalytic and regulatory subunits. F and G, in vitro dephosphorylation of TFEB (G) or TFE3 (H) with recombinant purified PP2A subunits.