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. 2018 Jun 20;293(32):12394–12404. doi: 10.1074/jbc.RA118.003441

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© 2018 Skotnicová et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 4. — Separation of the purified HemJ.f protein by size-exclusion chromatography. A, the HemJ.f pulldown obtained from the ΔPSI genetic background was loaded on a size-exclusion chromatography column, and eluted proteins/complexes were detected by absorbance 280 and 415 nm. Positions of standards are shown at the top of the graph: PSII[2], PSII dimer (600 kDa); PSII[1], PSII monomer (300 kDa); β-AM, β-amylase (200 kDa); ADH, alcohol dehydrogenase (150 kDa); BSA, BSA (66 kDa); DDM, micelle of dodecyl-β-maltoside; 3xFLAG, 3× FLAG peptide used to elute HemJ.f from the anti-FLAG-M2 agarose resin. B, absorption spectrum of 9.4 ml size-exclusion chromatography fraction was recorded by a HPLC diode-array detector.