Figure 5.

One-dimensional SDS–PAGE and two-dimensional CN/SDS–PAGE separation of HemJ.f eluate. A, proteins isolated by affinity chromatography from HemJ.f strain and from WT control cells were separated by 12 to 20% SDS–PAGE and stained with CBB, and the individual proteins bands were identified by MS (Table S1). B, the gel strip from CN–PAGE (see Fig. 1) was further separated in a second dimension by 12–20% SDS–PAGE and stained with SYPRO Orange. HemJ.f bands (marked as CN1 and CN2 in Fig. 1) were tentatively assigned as dimeric (HemJ[2]) and tetrameric (HemJ[4]) HemJ.f oligomers, respectively.