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ITC determination of l-captopril/di-zinc NDM-1 dissociation constant. A representative experiment is shown for ITC of NDM-1 and l-captopril, with the thermograms and fits for the other NDM variants included in Fig. S4. Values for Kd of all variants are given in Table S5. Fits above give Kd and stoichiometry for di-zinc NDM1 and l-captopril binding as 4 ± 1 μm and 1.0 ± 0.1, respectively.
