Figure 4.

Biochemical analysis of scaffolds after 4 weeks in culture. (a) Höchst 33528 dye was used to quantify the DNA in proteinase K digests of scaffolds. (b) Dimethylmethylene blue (DMMB) was used to determine the total amount of sulphated glycosaminoglycan (GAG) produced by mesenchymal stem cells (MSCs) from both the collected culture media and proteinase K scaffold digests. (c) The GAG/DNA ratio was calculated from total DNA and GAG values to show the production of GAG relative to the MSCs present in each group. Error bars represent standard deviation. Statistical significance was defined as P ≤ 0.05; *P ≤ 0.05, **P ≤ 0.001 and ***P ≤ 0.0001