Table 4.
Steady-state Kinetic Constants for Long-Chain Acyl-CoA Substrates with Tryptamine as the Acyl Acceptora,b
| Acyl-CoA | Km,app (μM) | kcat,app (s−1) | (kcat/Km)app (M−1 s−1) |
|---|---|---|---|
| Lauroyl-CoA | 0.97 ± 0.12 | 1.3 ± 0.01 | (1.4 ± 0.2) × 105 |
| Myristoyl-CoA | 0.92 ± 0.20 | 0.66 ± 0.01 | (7.2 ± 1.5) × 105 |
| Palmitoyl-CoA | 1.1 ± 0.30 | 0.51 ± 0.01 | (4.9 ± 1.4) × 105 |
| Oleoyl-CoA | 1.7 ± 0.51 | 0.42 ± 0.02 | (2.4 ± 0.70) × 105 |
| Arachidonoyl-CoA | 1.2 ± 0.67 | 0.27 ± 0.02 | (2.4 ± 1.3) × 105 |
a
Reaction conditions were 300 mM Tris pH 8.0, 150 μM DTNB, 60 mM tryptamine, and varied initial concentrations of the acyl-CoA.
b
Kinetic constants are reported with the standard error (n = 3).