Fig. 3.

S-acylation is important for the effect of C4 on Arabidopsis development. (A) Seedlings of estradiol-inducible pER8-C4^WT (wild-type) and pER8-C4^C8S (C8 mutated from cysteine to serine) plants germinated on media with (bottom panel) or without (top panel) 2 µM estradiol for 6 d. Scale bars are 1 cm. (B) Shoot phenotypes of control, pER8-C4^WT, and pER8-C4^C8S plants germinated on a medium containing 2 µM estradiol. (C) Seedlings were germinated and grown on regular medium for 7 d, then transferred onto a medium containing 2 µM estradiol for 3 weeks. The images are from independent experiments using individual transgenic lines, which produced similar patterns. (D) Agrobacteria carrying pCanG-MYC (Vector), pCanG-C4^WT-MYC, or pCanG-C4^C8S-MYC were injected into Nicotiana benthamiana leaves. After 4 d, their phenotypes were observed and the levels of C4 proteins were detected with using immunological blotting with an anti-MYC antibody. Coomassie Blue staining of total proteins was used as a loading control. WT, wild-type. All results in this figure are representative of at least three independent experiments.