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. 2018 Jun 20;69(18):4459–4468. doi: 10.1093/jxb/ery228

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© The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 6. — C4-induced misexpression of WUS is dependent on its S-acylation. (A) GUS staining levels of WUSpro::GUS in the estradiol-inducible pER8-C4^WT (wild-type) and pER8-C4^C8S (C8 mutated from a cysteine to a serine) plants 5 d after germination on media with (estradiol) or without (DMSO) induction by estradiol. Scale bars are 0.5 mm. (B) GUS staining levels of CLV3Pro::GUS in the pER8-C4^WT and pER8-C4^C8S plants 5 d after germination on media with or without estradiol. Scale bars are 0.5 mm. The results are representative of three independent experiments using individual transgenic lines, which had similar patterns. (C) Representative siliques of Arabidopsis infected with the geminivirus carrying the wild-type (WT) or C8S version of C4. The empty vector was used as an inoculation control.