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Germination percentage and RNA quality of seed lots used for RNA sequencing
| Harvest year | Germination (%) | RNA yield (µg RNA mg−1 tissue) | RIN | mRNA yield (ng poly(A)-selected RNA mg−1 tissue) | |
|---|---|---|---|---|---|
| 2016 | 2017 | ||||
| 2015 | 100 | 100 | 15.29 ± 2.49 | 7.86 ± 0.34 | 22.62 ± 5.67 |
| 1994 | 61 | 0 | 11.31 ± 1.04* | 6.72 ± 0.08** | 15.94 ± 7.68 |
Germination was measured in 2016 and 2017. RNA data show the mean ±SD (n=5) for RNA extracted from individual embryonic axes. *P<0.05, **P<0.0001 (Student’s t-test).
