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. 2017 Nov 21;24(1):2–13. doi: 10.1093/molehr/gax061

Figure 1.

Figure 1

Expression of CYP27A1 mRNA during luteolysis in rhesus macaques and sheep, and regulation of CYP27A1 mRNA by LH and hCG. Panel (A) shows normalized microarray mRNA expression of CYP27A1 in rhesus macaque CL collected during the luteal phase (ML = mid-late, Days 10–12; FL = functional late, Days 14–16 and P4 > 1.5 ng/ml; FRL = functionally regressed late, Days 14–16 and P4 < 0.5 ng/ml; VL = very late, menses, Days 18–19). Asterisks denote significant differences between groups indicated by brackets. Panel (B) displays CYP27A1 mRNA expression normalized to MRPS10 during PGF2α-induced and spontaneous luteolysis in the ovine CL. The x axis is separated between the induced and spontaneous luteolysis models. Asterisks denote significant differences between saline and PGF2α treatments at the corresponding time point (induced luteolysis), or between mid (Days 9–10) and late (Days 14–16 with serum P4 < 1 ng/ml) luteal phase CL (spontaneous luteolysis). Panel (C) is the effect of LH on CYP27A1 mRNA in rhesus macaques. Antide is a gonadotropin releasing hormone antagonist. The asterisk denotes a significant difference from all other groups. Panel (D) is the effect of hCG on CYP27A1 mRNA in human luteinized granulosa cells. Data are displayed as the fold change relative to the no hCG, 1 Day, group. Asterisks denote a significant difference between treatments on the corresponding day. For all panels, error bars indicate one standard error of the mean (SEM); *P < 0.05, **P < 0.01, ***P < 0.001.