Figure 3.

The effects of pharmacologic inhibitors of steroidogenesis and steroid replacement on mRNA expression of genes involved in SREBP2 and LXR-mediated cholesterol metabolism in human luteinized granulosa cells. Panel (A) displays QPCR data for CYP27A1, SREBF2, and both LXR isoforms. Panel (B) displays the SREBP2 target genes LDLR and HMGCR, as well as the LXR target gene ABCA1. For all charts, data are displayed as the fold change relative to DMSO. Error bars indicate one SEM, and asterisks indicate significant differences between groups connected by brackets; **P < 0.01, ***P < 0.001. Neither dose of P4 replacement significantly altered the mRNA expression of any gene relative to the aminoglutethimide-only treatment.