Table I.
Primer and Taqman MGB probe sequences used in QPCR analyses.
| Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) | MGB probe (5′–3′) or SYBR |
|---|---|---|---|
| Human sequences | |||
| ABCA1 | TCCAGGCCAGTACGGAATTC | TCCTCGCCAAACCAGTAGGA | CTGGTATTTTCCTTGCACCAA |
| ABCG1 | GACCAGCTTTACGTCCTGAGTCA | GTTCAGACCCAAATCCCTCAAAT | AAAGTCTGCAATCTTGTGCC |
| LDLR | CTGGTCAGATGAACCCATCAAAG | GCCGATCTTAAGGTCATTGCA | CCAACGAATGCTTGGAC |
| NR1H2 | ATCGTGGACTTCGCTAAGCAA | GATCTCGATAGTGGATGCCTTCA | TGCCTGGTTTCCTGC |
| NR1H3 | TCCCCATGACCGACTGATG | CAGACGCAGTGCAAACACTTG | TCCCACGGATGCTAAT |
| MRPS10 | TTCCAAAGGATTTGACCAAACC | TCGTGACCTTTCACCAAAACC | ATCTCTGATGAACCAGACAT |
| CYP27A1 | GGCCAAGTACGGTCCAATGT | CGCATCACTTGCTCCAAGAG | SYBR Green |
| HMGCR | AACACGATGCATAGCCATCCT | AAGGCCAGCAATACCCAAAA | SYBR Green |
| STAR | GTGGGTGCCTTCCAGAAATATAGT | TGACTGGTGCCTATGAAAGCAA | SYBR Green |
| SREBF2 | ATCGCTCCTCCATCAATGACA | TCCTCAGAACGCCAGACTTGT | SYBR Green |
| G6PD | GAAGCCGGGCATGTTCTTC | TAGGCGTCAGGGAGCTTCAC | SYBR Green |
| Ovine sequences | |||
| CYP27A1 | TGGAGTAGACACGACATCCAACA | ACGCCCACCACTTCCTTATG | CCCTGTACCATCTTTCAA |
| MRPS10 | TTGAGCTCTCGAGAAATCACTGAA | CTGGGTGCAAACCTAACAAAAGA | ATTGGTTCCTTCATCCTGT |