Fig. 7.

In vivo characterization of FhTPS genes. FhTPS genes together with p19 were transiently expressed in tobacco leaves by Agrobacterium-mediated infiltration; the corresponding ectopic proteins could use the tobacco endogenous substrate to generate volatile terpenes. The products produced in tobacco leaves on the fifth day after transformation were analyzed by GC-MS. New product peaks were observed in contrast to controls and were identified by comparing mass spectra with the NIST 2008 mass spectra library. The mass spectra of products, e.g. linalool and α-terpineol, are shown in Supplementary Fig. S19. Leaves of tobacco infiltrated by p19 alone were used as a control. The X-axis represents the retention time of the peak outflow, and the Y-axis represents the integrated area of the chromatographic peak.