Table 4.
Effect of protein supplementation (treatment), fraction, sub-species (Bos taurus and Bos indicus), and time (0 h and 4 h postfeeding) on the relative abundance of the most abundant phyla
| Phylum1 | Relative abundance | Fraction P-value | Fraction | |
|---|---|---|---|---|
| Liquid | Solid | |||
| Bacteroidetes5 | 65.44 | <0.0001 | 70.00 | 60.80 |
| Firmicutes2,5 | 28.13 | <0.0001 | 23.40 | 32.90 |
| Spirochaetes3,5 | 2.22 | <0.0001 | 1.90 | 2.50 |
| Fibrobacteres3 | 1.14 | >0.05 | ||
| Proteobacteria5 | 0.94 | <0.0001 | 1.40 | 0.50 |
| Tm7 | 0.55 | 0.01 | 0.51 | 0.59 |
| Tenericutes | 0.48 | <0.0001 | 0.79 | 0.17 |
| Chloroflexi5 | 0.2 | <0.0001 | 0.09 | 0.31 |
| Actinobacteria | 0.19 | <0.0001 | 0.11 | 0.27 |
| Synergistetes | 0.18 | <0.0001 | 0.11 | 0.24 |
| Verrucomicrobia4 | 0.17 | >0.05 | ||
1No significant sub-species × treatment or fraction × treatment differences.
2Treatment (P = 0.025) Control = 27.2%, 50 L UIP = 29.1%, 150 L UIP = 27.8%, H UIP = 29.5%, and 150 H UIP = 27.1%, CON= supplemented, 50 H DIP = 50 mg N/kg BW 69.5% wheat middling, 30% soybean meal, and 0.5% urea, 150 H DIP = 150 mg N/kg BW 69.5% wheat middling, 30% soybean meal, and 0.5%, 50 L DIP = 50 mg N/kg BW 100% distillers’ grains, 150 L DIP = 150 mg N/kg BW 100% distillers’ grains.
3Fraction × sub-species (P < 0.05), Spirochaetes: Angus × liquid 1.67%, Brahman × liquid 2.15%, Angus × solid 2.63%, Brahman × solid 2.44% and Fibrobacteres: Angus × liquid 0.89%, Brahman × liquid 1.25%, Angus × solid 1.29%, Brahman × solid 1.16%.
4Sub-species (P = 0.01), Angus 0.19% vs. Brahman 0.13%.
5Time (P < 0.006) Bacteroidetes 0 h 66.7% vs. 4 h 64.1%, Firmicutes 0 h 27.4% vs. 4 h 29.0%, Spirochaetes 0 h 2.0% vs. 4 h 2.4%, Proterobacteria 0 h 0.9% vs. 4 h 1.0%, Chloroflexi 0 h 0.15% vs. 4 h 0.24%.