Table 7.
Functional analysis via PICRUSt of the most abundant pathways in the solid and liquid fraction of the rumen in Bos taurus and Bos indicus steers (n = 5) fed a low-quality forage
| Level 2 KEGG gene pathway | % Solid | % Liquid | % Difference |
|---|---|---|---|
| Replication and repair* | 10.89 | 9.64 | 12.2 |
| Amino acid metabolism* | 10.26 | 9.94 | 3.2 |
| Carbohydrate metabolism* | 10.38 | 9.21 | 11.9 |
| Membrane transport* | 8.61 | 6.83 | 23.1 |
| Translation* | 7.67 | 6.84 | 11.4 |
| Metabolism of cofactors and vitamins* | 4.93 | 4.4 | 11.4 |
| Glycan biosynthesis and metabolism | 3.38 | 3.27 | 3.3 |
| Folding, sorting, and degradation* | 3.48 | 3.14 | 10.3 |
| Lipid metabolism* | 2.73 | 2.41 | 12.5 |
| Transcription* | 2.29 | 1.83 | 22.3 |
| Metabolism of terpenoids and polyketides* | 1.76 | 1.61 | 8.9 |
| Metabolism of other amino acids* | 1.64 | 1.51 | 8.3 |
| Signal transduction* | 1.6 | 1.3 | 20.7 |
| Cell motility* | 1.78 | 1.06 | 50.7 |
| Biosynthesis of other secondary metabolites* | 1.17 | 1.07 | 8.9 |
| Xenobiotics degradation and metabolism* | 1.16 | 1.04 | 10.9 |
| Signaling molecules and interaction | 0.55 | 0.54 | 1.8 |
| Transport and catabolism | 0.49 | 0.5 | 2.0 |
*Indicates P value < 0.05 based on Kruskal–Wallis analysis of variance with Bonferroni corrections.