Table 2.
Day | Treatment2 | Total sequences | Subsampled to 4,618 sequences | ||
---|---|---|---|---|---|
Chao13 | Shannon4 | Chao13 | Shannon4 | ||
0 | CTL | 4,232 | 4.82 | 1,178 | 4.68 |
SDPP | 9,197 | 5.73 | 1,625 | 5.52 | |
7 | CTL | 3,289 | 5.02 | 1,410 | 4.90 |
SDPP | 5,113 | 5.78 | 1,673 | 5.67 | |
14 | CTL | 6,108 | 5.26 | 1,522 | 5.10 |
SDPP | 4,451 | 5.01 | 1,069* | 4.90 | |
21 | CTL | 4,233 | 4.29 | 1,202 | 4.16 |
SDPP | 8,175 | 3.77 | 929 | 3.64 | |
28 | CTL | 4,794 | 3.74 | 963 | 3.65 |
SDPP | 4,233 | 3.28 | 790 | 3.17 | |
SEM | – | – | 118.1 | 0.35 | |
Statistics | P, treatment | – | – | 0.60 | 0.70 |
P, day | – | – | <0.001 | <0.001 | |
P, treatment × day | – | – | 0.001 | 0.106 |
1Total number of processed sequences was 2,685,690. The average number of sequences per sample was 38,367. Number of sequences of each sample was standardized to 4,618, which is the lowest number of sequences of a sample. The normalized values were used for statistical analysis.
2Treatment included control (CTL) and SDPP.
3Chao1 measures the microbial richness. It is estimated by the number of observed species divided by the ratio of singletons and doubletons.
4Shannon measures the microbial richness and evenness.
*P < 0.01: comparison between SDPP and CTL group at the corresponding time.