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. 2018 Aug 15;13(8):e0200955. doi: 10.1371/journal.pone.0200955

Fig 3. Gene targeting is stimulated by HHV1 ICP8 and inhibited by phage λ Beta in human cells.

Fig 3

Cells were incubated with doxycycline to induce synaptase expression and subsequently transfected with 50 nM of oligo 85. A) The background of green cells in “no oligo” control experiments (0.001% for pSLIK4, 0.003% for pSLIK5 and 0.012% for pSLIK6) was subtracted from recombination frequencies. B) Recombination frequencies in (A) were normalized to the mean of the endogenous recombination frequencies in the pSLIK6 control line. C) Cells were gated for Crimson expression (which is expressed in equimolar amounts with the synaptase via translational coupling through a P2A linker) and evaluated as in (A). D) Recombination frequencies in (C) were normalized to the mean of the endogenous recombination frequencies in the pSLIK6 control line and evaluated as in (A). Data were evaluated by Fisher’s Exact Test of significance; *** indicates P<0.005 and **** indicates P<0.001. The figure shows sample data from one of eight biological replicates, n = 12 trials, and error bars reflect SEM.