Fig 2. The anti-ferroptotic activity of vitamin E and its metabolites.
(A) Ferroptosis was induced in Q7 cells by RSL3 treatment (2 μM 18 h), as measured by CellTiter-Glo® 2.0 ATP assay. The dose-dependent cytoprotective activity of vitamin E (αT), its metabolites (αTQ, αTHQ), or the synthetic carbochroman (αTCC) was assessed by co-treatment (in 24-point titration) with RSL3. Curves are representative of ≥9 independent experiments, of which 7 evaluated αTCC to a top concentration of 30 μM. (B) Ferroptosis was initiated in BODIPYTM 581/591 C11-prelabeled Q7 cells by RSL3 (2 μM) treatment, with cellular lipid oxidation assessed by the rate of change in green cellular fluorescence using time-lapse microscopy. Rescue compounds were co-treated (in 6-point titration) with RSL3. Mean ± SEM shown, (n = 6 to 9 technical replicates for each condition. Results are representative of 2 independent experiments.