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. 2018 Aug 15;13(8):e0201369. doi: 10.1371/journal.pone.0201369

Fig 5. Lipid changes quantitated in RSL3-treated Q7 cells or their culture medium.

Fig 5

(A) RSL3-Q7 treated cells have elevated cellular levels of a lyso-PE-palmitoyl species (*p<0.05, Unpaired t-test with Welch’s correction). (B, C) RSL3-treated Q7 cells have elevated cellular and conditioned media levels of 15-HETE, an effect prevented by αTQ (5 μM) co-treatment (*p<0.05, **p<0.01, ***p<0.001, Shapiro-Welk normality test followed by ANOVA with Sidak’s multiple comparisons test or Kruskal-Wallis test with Dunn’s multiple comparisons test, respectively). (D, E) 12-HETE levels were not altered by RSL3 or αTQ treatment (ns, p>0.05, Shapiro-Welk normality test followed by Kruskal-Wallis test with multiple comparisons test. (F, G) Conditioned medium of RSL3-treated Q7 cells has elevated AA, but cellular levels are not significantly changed (***p<0.001, Shapiro-Welk normality test followed by ANOVA with Sidak’s multiple comparisons test).