Fig 1. Bacterial composition of whole blood and its 20 × g plasma evaluated using metagenome sequencing and GSTK analysis.

DNA was extracted from control blood (A), gram-positive bacteria-spiked blood (B), gram-negative bacteria-spiked blood (C), or their respective plasma after clarification by 20 × g centrifugation. The numbers in the parenthesis to the right show the number of reads obtained from the sequencing run. The abbreviation are as follows: SA, Staphylococcus aureus; SP, Streptococcus pneumoniae; En, Enterococcus faecalis; PA, Pseudomonas aeruginosa; E, Escherichia coli; and HI, Haemophilus influenzae.