Fig 2. Bacterial composition identified by 16S rRNA gene amplicon sequencing followed by GSTK or Ion Reporter analysis of control and bacteria-spiked blood.

Seven variable regions of the 16S rRNA gene were amplified by PCR from either gram-positive bacteria-spiked whole blood (A) or gram-negative bacteria-spiked whole blood (B) used in Fig 1, and sequenced using Ion PGM. The sequence reads were analyzed using GSTK or Ion Reporter suite software.