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. 2018 Aug 3;14(8):e1007202. doi: 10.1371/journal.ppat.1007202

Fig 2. MHV-68 induces SOCS1 expression in BMMs.

Fig 2

(A) BMMs were infected with MHV-68 at MOI = 10. At 13 hpi, total RNA was extracted and the mRNAs levels of Socs1, Socs3, Socs6, Cis and Shp2 were determined by RT-qPCR. (B-C) BMMs were either infected with MHV-68 or UV-inactivated MHV-68. At the indicated hours post infection, the cells were collected for RT-qPCR and western blotting to evaluate the SOCS1 mRNA and protein expression levels. In addition, ORF6 DNA qPCR was performed to assess the extent of viral replication. SOCS1 qPCR data are expressed as fold change in mRNA level compared to that in uninfected cells. The results are representative of three independent experiments and are expressed as means ± S.E.M.