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. 2018 Aug 15;13(8):e0202446. doi: 10.1371/journal.pone.0202446

Table 4. Effects of TMR and SF system on ruminal fermentation characteristics1.

Time interval2 1.5 h 3 h 4.5 h SEM P-value
(FS)
P-value (Time)
Item/Feed type TMR SF TMR SF TMR SF 1.5 h 3 h 4.5 h
Volatile fatty acids                      
Total VFA (mM) 122.8 95.4 114.4 118.9 104.7 126.8 9.7 0.962 0.009 0.650 0.032
Acetate, % 55.0 58.4 56.0 54.0 58.6 55.3 1.2 0.337 0.007 0.103 0.008
Propionate, % 25.0 22.5 24.0 25.2 22.5 24.1 0.9 0.876 0.008 0.160 0.088
Butyrate, % 12.7 11.8 13.2 13.0 13.1 13.2 0.9 0.649 0.332 0.877 0.977
Isobutyrate, % 0.6 1.3 0.6 1.2 0.5 1.3 0.1 < .0001 < .0001 < .0001 < .0001
Valerate, % 1.9 1.4 2.0 1.8 1.7 1.9 0.1 0.048 < .0001 0.064 0.072
Isovalerate,% 4.7 4.5 4.1 4.6 3.4 4.2 0.2 0.027 0.352 0.110 0.006
Acetate: Propionate 2.2 2.6 2.3 2.2 2.6 2.3 0.1 0.921 0.001 0.124 0.038
NH3-N, mg/L 18.9 9.2 14.9 14.8 9.4 15.1 1.8 0.124 < .0001 0.925 0.005
pH 6.5 6.7 6.5 6.5 6.6 6.3 0.1 0.899 0.067 0.808 0.060

1 Values are LS means with standard error

2 Sampling time after morning feeding; TMR- Total Mixed Ration; SF- Separate Feeding; SEM- Standard Error of the Means

FS–Feeding system