| Protein concentration |
0.7 mg/ml |
| Sample volume for EM grid |
3 µl |
| Grid type |
UltraAuFoil 1.2/1.3 |
| Plunge freezer |
Leica GP EM |
| Blotting time |
10 s |
| Blotting temperature |
4°C |
| Blotting chamber humidity (set/measured) |
95%/88% |
| Microscope |
FEI Titan Krios |
| Camera |
K2 Summit |
| Energy Filter |
no |
| Cs corrector |
no |
| Magnification |
29,000x |
| Dose rate |
~4.5 e-/px ∙ s |
| Total dose |
~40 e-/Å2
|
| Number of frames |
38 |
| Physical pixel size (microscope) |
0.858 Å/px |
| Super-resolution pixel size (microscope) |
0.429 Å/px |
| Final pixel size used for final maps |
0.835 Å/px |
| Number of total micrographs |
3,085 |
| Number of selected micrographs |
2,062 |
| Number of particles picked |
281,021 |
| Number of particles selected containing a lipid nanodisc |
118,556 |
| Number of particles in α4β4 map |
47,482 |
| Number of particles in the cytosolic domain map |
57,384 |
| Number of particles in the TM domain map |
65,745 |
| Average resolution in α4β4 map |
3.3 Å |
| Average resolution in cytosolic domain map |
3.0 Å |
| Average resolution in TM domain map |
4.0 Å |
| B-factor for α4β4 map |
−89 |
| B-factor for cytosolic domain map |
−84 |
| B-factor for TM domain map |
−123 |
