Figure 4. Quantification of phospholipids trapped in peptidiscs.
(A) Calculated number of phospholipids per peptidisc. Phospholipid content was determined by Malachite green assay after acid digestion of lipid extracts. Error bars represent standard deviation derived from three separate measurements. B) TLC analysis of lipid extracts obtained from 10 µg MalFGK2 peptidisc, 10 µg FhuA peptidiscs and 20 µg BRC peptidiscs, as well as pure lipid standards Cardiolipin (CL), 1,2-dioleoyl-sn-glycero-3-phosphoglycerol (PG), and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (PE).