Table 2. Molecular weight, diameter, and scaffold stoichiometry of MalFGK₂ reconstituted in peptidisc.

	Measured molecular weight (kDa)*	Measured diameter †	Calculated stoichiometry (scaffold/disc)‡
MalFGK2 Peptidisc	250 ± 17	11.7 ± 1.4	12 (±2):1

*Molecular weight calculated from SEC-MALS data (Fig. S1). The standard error is derived from three independent SEC-MALS experiments. †Diameter of MalFGK₂-peptidiscs determined by negative stain electron microscopy Figure 1B, assuming a perfectly circular shape. ‡Stoichiometry (n), based on the measured diameter of the particles, was calculated with the following formula: π(d_disc-2d_α-helix) = (n/2)L_NSPr; where d_α-helix represents the diameter of an alpha-helix (0.5 nm), d_disc represents the measured disc diameter, and L_NSPr represents length of the NSP_r peptide.