Figure 4.

DCA inhibits TXB2 A2 production and ATP secretion in platelet response to collagen. The level of TXB2 A2 generation was measured in human (A, left) and mouse (B, left) RP and in platelet response to suboptimal dose of collagen (0.46 μg). PRP samples were preincubated in the presence or absence of DCA (10 and 25 mM), aspirin (100 μM), and indomethacin (20 μM). The aggregation was performed for 3 minutes, and the reaction was stopped by quickly freezing the sample in a dry ice–methanol bath. Samples were prepared, and the level of TXA2 was measured with an enzyme-linked immunosorbent assay kit. ATP secretion was measured by luciferase/luciferin-based reaction. Luminescence generated by secreted ATP from human (A, right) and mouse (B, right) platelets were monitored using a Lumi-aggregometer. Asp., aspirin; Ind., indomethacine; NS, nonsignificant.