Figure 6.
DCA inhibits platelet aggregates/thrombus formation in vitro. The analysis was done using flow chamber system from Bioflux Microfluidics. Human (A) and mouse (B) whole blood pretreated with 50 mM DCA (lower channels) or control (upper channels) was perfused over a collagen-coated (150 μg/mL) surface for 10 minutes at a shear rate of 1500 s−1. Left panels show representative images taken using a 10× objective. Right panels show quantification of the surfaced area covered by fluorescent platelets after 10 minutes of perfusion. Four areas from different areas of flow chamber were analyzed from each blood sample. Data represent the mean percentage of surface area covered by fluorescence platelets ± SEM, with n = 5 per group for mice and 3 per group for the human.