Figure 4.

Erythroid lineage–specific character of the transcriptional activity of rs311103[G] genomic region. (A) Transcriptional activity levels of the reporter constructs bearing different genotypes at SNP rs311103 in K-562 cells. The reporter constructs 200-G and 200-C carry the 200-bp genomic regions, which encompass rs311103; the rs311103 sites of these constructs bear the G and C nucleotides, respectively. Similarly, the 62-G and 62-C reporter constructs carry the 62-bp genomic regions that encompass rs311103[G] and rs311103[C], respectively. The relative luminescence measurements obtained from each experiment were normalized against the level of β-galactosidase activity in the same cells. The results are presented as fold changes in the mean of luminescence units obtained from triplicate experiments relative to that obtained from the empty pGL4.23 reporter vector (p4.23); the error bars indicate the standard deviations. (B) The erythroid lineage–specific character of the transcriptional activity of rs311103[G] genomic region. The 62-G and 62-C reporter constructs and the empty pGL4.23 vector were transfected into human erythroleukemia cell lines HEL and LAMA-84 and into human embryonic kidney cell line HEK293T and human colon cancer cell line DLD-1.