Figure 7.

Association of the GATA1 factor with the SNP rs311103 genomic region in vivo. The association status of the endogenous GATA1 and GATA2 transcription factors with the rs311103 genomic region in HEL erythroleukemia cells was examined using ChIP analysis. The amount of the genomic segment spanning rs311103 present in the chromatin DNA immunoprecipitated with anti-GATA1 antibody, anti-GATA2 antibody, or normal rabbit immunoglobulin G (IgG) and the input DNA samples were quantitatively compared using real-time PCR (left panel). The results were obtained from 3 independent experiments and are presented as percentages of the amount of the rs311103 segments in the input DNA sample; the error bars indicate the standard deviations. The statistical differences between GATA1 and IgG and between GATA2 and IgG were analyzed using the unpaired t test. A gel image of a 2.0% agarose gel electrophoresis analysis of the PCR products is shown (right panel). **P < .01, *P < .05.