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. 2018 Aug 13;2(15):1985–1997. doi: 10.1182/bloodadvances.2018021113

Figure 2.

Figure 2.

PD-1, PD-L1, and PD-L2 expression in WM BM cells. BM biopsies from WM patients were processed to isolate cells (CD19+/138+ and CD19−/138+) and plasma fractions. Cells were used for RNA extraction, cDNA synthesis, and RT-PCR analysis. (A) Bar graphs represent relative gene expression of PD-1 and the ligands normalized to housekeeping gene; CD19−/138− (NBM, n = 9; WM, n = 18) and CD19+/138+ (NBM, n = 8; WM, n = 15). Significant differences and related P values are shown on each graph. (B) IHC staining shows the sections from WM and normal BM (NBM) stained for PD-1, PD-L1, and PD-L2 (brown stain; original magnification ×400). (C) CD19+/138+ and CD19−/138− cell populations were stained with PD-L1 and analyzed using flow cytometry. Histograms show PD-L1 surface expression on CD19+/138+, CD3+, CD56+, CD14+ and CD15+ cell fractions (isotype control [red] and PD-L1 [blue]) in WM and NBM. Bar graphs compare mean fluorescence intensity of 19+/138+ cells between NBM and WM BM (NBM, n = 5; WM, n = 9). Data are presented as mean ± SEM and significant differences and related P values are shown on each graph. MFI, mean fluorescence intensity.