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. 2018 Aug 9;2(15):1923–1934. doi: 10.1182/bloodadvances.2018018986

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Figure 1. — Spherical shape-inducing effects of IAIPs on purified human neutrophils. The neutrophils were incubated with HSA (1 μM), BSA (1 μM), or IAIPs (0-400 μg/mL) for 1 hour and the neutrophil shape was observed under a fluorescence microscope. (A-B) The rounding and size of neutrophils were analyzed by the In Cell Analyzer 2000. Total cell area (A) (µm²) and form factor (B) were determined. The results shown are the means ± SEM of 7 experiments. **P < .001 vs 0 μg/mL of IAIPs; ##P < .001 vs HSA and ††P < .001 vs BSA. (C) Typical picture of fluorescence staining from each group is shown (calcein stain; scale bars, 20 μm).