Skip to main content
. Author manuscript; available in PMC: 2019 Aug 15.
Published in final edited form as: ACS Chem Neurosci. 2018 Mar 27;9(8):2041–2053. doi: 10.1021/acschemneuro.7b00513

Figure 1.

Figure 1

Aplysia SPTR precursor and alignment of apSPTR-GF-DP2 with related sequences in other species. (a), Cleavage and processing of the Aplysia SPTR precursor. The sequence in green represents the signal peptide. Confirmed peptide sequences are underlined. The sequence in red represents apSPTR-GF-DP1. The sequence in orange represents the linker peptide. The sequence in blue represents apSPTR-GF-DP2. The confirmed amidation site is in bold, and an intramolecular disulfide bridge is confirmed as shown. The amino acids highlighted in yellow represent the cleavage sites of endopeptidase for the precursor. (b), Alignment of apSPTR-GF-DP2 with 16 C-terminal neuropeptides from SPTR-gene family precursors from 11 molluscs and 5 annelids using Bioedit. Note that all of the C-terminal neuropeptides have two cysteines, which may form a disulfide bridge. Not all of the C-terminal neuropeptides are amidated. *, represents amidated C termini which have been verified by MALDI; #, represents potential amidated C termini; peptides not labeled represent potential nonamidated C termini.