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. 2018 Jul 5;5(8):935–951. doi: 10.1002/acn3.592

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© 2018 Lankenau Institute for Medical Research. Annals of Clinical and Translational Neurology published by Wiley Periodicals, Inc on behalf of American Neurological Association.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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The 5F5 and 2G6 mAbs bind GluN1 on rat hippocampal neurons. (A) Neurons were cultured for 14 days and stained with ANRE CSF or mAbs (red). Top left, 5F5. Top right, 2G6. Bottom left, CSF. Bottom right, 8E1. Nuclei were stained with DAPI. Scale bar = 10 μm. (B) Neurons were stained with ANRE CSF or mAbs (red), and costained with murine anti‐GluN1 antibody (green). Rows: Top, 5F5. Middle, 2G6. Bottom, CSF. Columns: Left, GluN1. Middle, CSF or mAbs. Right, Merged images. Nuclei were stained with DAPI. Scale bar = 10 μm.