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Internalization of the 5F5 and 2G6 mAbs by hippocampal neurons and the effects of MK‐801 and AP5. (A) Rat hippocampal neurons were incubated with 5F5, 2G6, or 6A mAbs conjugated to the pH‐sensitive fluorescent dye, CypHer5E, which is activated by the low pH in endosomes, alone and in the presence of MK‐801 or AP5. (B) Neurons treated with MK‐801 or AP5 were assessed for binding of the 5F5, 2G6, or 6A mAbs. Scale bar = 5 μm.
