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. 2018 Aug 15;8:12236. doi: 10.1038/s41598-018-30362-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of CRELD2 in Neuro2a cells. Neuro2a cells were treated with or without thapsigargin (Tg, 0.1 μM), tunicamycin (Tm, 1 μg/ml), breferdin A (BFA, 2.5 μg/ml) or serum-deprived medium (serum free, SF) for 8 h (A) and 12 h (B). (C) Neuro2a cells under the serum-deprived medium were treated with Tg, Tm, BFA or vehicle for 12 h. After treatment, secreted CRELD2 (medium) and the indicated proteins in each cell lysate (lysate) were prepared as described in the Materials and methods section. The expression of the indicated mRNA (A) and protein (B,C) was detected by RT-PCR and western blotting analysis as described in the Materials and methods section. Representative data from more than three independent experiments are shown.