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. 2018 Aug 15;8:12236. doi: 10.1038/s41598-018-30362-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Long term treatment with tunicamycin induced CRELD2 protein in Neuro2a cells. Neuro2a cells in serum-containing culture medium (A) or serum-deprived medium (B) were treated with tunicamycin (Tm, 1 μg/ml) for the indicated time. Expression of the indicated protein in each culture medium and cell lysate was detected as described in the Materials and methods section, and reproducibility was confirmed.