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. 2018 Aug 15;3:20. doi: 10.1038/s41525-018-0062-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Validation of high-throughput glucose production assay. The glucose production assay of primary hepatocytes was validated in 12 96-well plates (1152 wells in total) to assess assay variability. Concentrations of insulin were titrated as indicated in the figure. The X-axis indicates well number and the Y-axis indicates raw signal value of absorbance at 570 nm