Fig. 2.

Genetic dependencies of PEL cell lines. a Significance of dependency of all genes screened by Brunello library in 8 PEL cell lines. Genes are ranked using the median adj. p value scores (FDR). A large majority of genes, including those involved in NF-κB (e.g., RELA, NFKB1, and IKBKG) and cytokine signaling (e.g., STAT3 and JAK1), score as dispensable in PEL cells. Genes in yellow are considered housekeeping genes, non-housekeeping genes are in pink. Ranks among PSODs are in parentheses. b Workflow and criteria for classifying “housekeeping genes” and “PEL gene dependencies”, based on CRISPR screens in this study and 52 publicly available screens. PEL gene dependencies that do not have housekeeping functions are further considered “PEL-specific oncogene dependencies” (PSODs). c Pathway enrichment analysis of PSODs using DAVID for gene sets from GO (orange) or KEGG (blue). Number of genes included in each enriched pathway is indicated. Full results are in Supplementary Data 11