Fig. 6.

The levels of Viking and Pericardin in aged SPARC heterozygous hearts.

(A) Confocal slices through the distal region of the hearts of young (1 week) and older (6 week) control (w¹¹¹⁸) and SPARC heterozygous flies (SPARC^MI00329) stained with antibodies to Pericardin. (B) Mean (± SEM) Pericardin fibre thickness in flies of different ages and genotype as described in the figure. ns = P > 0.05. n = 6 measurement from 4 four individual flies. (C) Mean (± SEM) of the Viking-GFP signal at the valves of flies of different ages and genotype (w¹¹¹⁸, black lines and SPARC^MI00329, red lines). ns = P > 0.05. n = 6–10 flies of each age and genotype. (D) Western blot of heart tissue stained with Pericardin antibodies and actin as a loading control. (E) Quantification of anti-Pericardin western blot band intensity using at one week of age; ns = not statistically different from control. (F) Quantified data showing fold change in Pericardin band intensity; n = three blots; *P < 0.05 for there being a difference in Pericardin at 6 weeks compared to 1 week.