FIG 11.
OGT inhibition enhances antitumorigenic activity of a proteasome inhibitor. (A) Immunoblot analysis of O-GlcNAced proteins and OGT in H460 cells expressing doxycycline (Dox)-inducible control shRNA or OGT shRNAs. Whole-cell extracts were prepared at 24 h after treatment with 1 μg/ml doxycycline. Tubulin was used as a loading control. (B and C) Effects of bortezomib (BTZ) on the tumor growth of H460 cells expressing control or OGT shRNA in xenograft mice model. A total of 1 × 106 H460 cells were subcutaneously transplanted into nude mice. Fifteen days after the transplantation, bortezomib was directly administered into each tumor twice. (B) Tumor volume was calculated on 3 and 6 days after the initial treatment of bortezomib. (C) Fold changes of tumor volumes on day 3 and day 6 were calculated against that on day 0. *, P < 0.05. n.s., not significant.