FIG 4.

OGT/HCF-1 complex is required for chromatin binding of NRF1. (A) NRF1 ChIP-seq binding sites in PSMA5, PSMD11, PSMD14, and GATA-1 gene loci. An arrowhead indicates the position of a primer set that was used for the ChIP assay within each target gene locus. (B) Quantitative ChIP assay at each gene locus in HeLa cells that were treated with control siRNA, two distinct siRNAs specific for HCF-1 (HCF-1-si1 and HCF-1-si2), or two distinct siRNAs specific for NRF1 (NRF1-si1 and NRF1-si2). Chromatin localization of NRF1 was examined in each sample that was treated with 1 μM MG132 or DMSO for 4 h. The values show the enrichment of immunoprecipitated DNA relative to input DNA. Averages and SDs were calculated from triplicate samples. *, P < 0.01; **, P < 0.001.