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. 2018 Aug 15;38(17):e00252-18. doi: 10.1128/MCB.00252-18

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Copyright © 2018 Sekine et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 6 — Hexosamine biosynthesis pathway is required for accumulation of NRF1 protein. (A and B) NRF1 accumulation in response to MG132 treatment in HeLa (A) and MDA-MB231 (B) cells. HeLa and MDA-MB231 cells were pretreated with 100 μM DON for 24 h and treated with 1 μM MG132. Whole-cell extracts were prepared at the indicated time points after the cells were treated with MG132. Tubulin was used as a loading control. (C and D) NRF1 accumulation in response to b-AP15 treatment in MDA-MB231 cells. At 72 h after transfection with OGT siRNA (C) or 24 h after 100 μM DON treatment (D), whole-cell extracts were prepared at the indicated time points after treatment with b-AP15. Tubulin was used as a loading control.